US 7,463,358 B2
	Highly stable surface plasmon resonance plates, microarrays, and methods
Nick Wolf, Covington, Wash. (US); Danliang Jin, Bothell, Wash. (US); Anna M. Barklund, Kirkland, Wash. (US); and Raluca Dinu, Redmond, Wash. (US)
Assigned to Lumera Corporation, Bothell, Wash. (US)
Filed on Dec. 06, 2005, as Appl. No. 11/296,659.
Prior Publication US 2007/0128455 A1, Jun. 07, 2007
Int. Cl. G01N 21/55 (2006.01)

U.S. Cl. 356—445  [356/448; 435/287.7; 435/287.8; 435/288.7; 428/446; 428/447; 428/450; 428/615; 428/621; 428/624; 428/629; 428/630; 428/668]

45 Claims

36. A method of detecting a biomolecule, comprising a) providing an article including: i) a substrate; ii) a first metal film overlying the substrate; iii) a second thin metal film overlying the first metal film; iv) an organosilane covalently bonded to the exposed upper surface of the second thin metal film through metal-oxygen-silicon bonds; and v) a binding group attached to the organosilane; and b) determining whether or not the biomolecule is bound to the binding group by detecting or measuring a surface plasmon resonance signal generated from the first metal film.