| US 7,462,470 B2 | ||
| Method for the producing vanillic acid and vanillin from waste residue of rice bran oil by fermentation and biotransformation | ||
| Zhihao Sun, Wuxi (China); Pu Zheng, Wuxi (China); Xinfu Guo, Hangzhou (China); Guanyu Lin, Hangzhou (China); Hanghua Yin, Hangzhou (China); Jun Wang, Hangzhou (China); and Yanbing Bai, Hangzhou (China) | ||
| Assigned to Zhejiang Hangzhou Xinfu Pharmaceutical Co., Ltd, Zhejiang (China) | ||
| Filed on Jan. 27, 2006, as Appl. No. 11/340,845. | ||
| Claims priority of application No. 2005 1 0077191 (CN), filed on Jun. 17, 2005. | ||
| Prior Publication US 2006/0292676 A1, Dec. 28, 2006 | ||
| Int. Cl. C12P 7/24 (2006.01); C12P 7/42 (2006.01) | ||
| U.S. Cl. 435—146 [435/136; 435/147; 435/254.1; 435/254.3] | 5 Claims |
| 1. A method for the producing vanillic acid and vanillin comprising:
(1) culturing Aspergillus niger CGMCC 0774 in a fermentor;
(2) adding waste residue of rice bran oil containing ferulic acid to the fermentation broth of step (1) to obtain a converted
liquid containing vanillic acid;
(3) filtrating the converted liquid of step (2) with a membrane, and autoclaving after supplementation with glucose;
(4) culturing Pycnoporus cinnabarinus CGMCC 1115 or a mutant thereof in a fermentor to obtain wet mycelium;
(5) mixing the wet mycelium of step (4) with the converted liquid of step (3) to perform a conversion reaction to produce
a solution of vanillin; and
(6) extracting vanillin from the solution of vanillin of step (5).
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