| US 7,593,819 B2 | ||
| Internal calibration standards for electrophoretic analyses | ||
| Timothy Hunkapiller, Mercer Island, Wash. (US); Cheryl Heiner, La Honda, Calif. (US); Curtis Gehman, Pasadena, Calif. (US); James Labrenz, San Francisco, Calif. (US); and Shiaw-Min Chen, San Jose, Calif. (US) | ||
| Assigned to Applied Biosystems, LLC, Carlsbad, Calif. (US) | ||
| Filed on Jul. 11, 2002, as Appl. No. 10/193,776. | ||
| Claims priority of provisional application 60/304934, filed on Jul. 11, 2001. | ||
| Prior Publication US 2003/0032042 A1, Feb. 13, 2003 | ||
| Int. Cl. G01N 33/50 (2006.01); C12Q 1/68 (2006.01) | ||
| U.S. Cl. 702—20 [435/6] | 1 Claim |
| 1. A program storage device readable by a machine, embodying a program of instructions executable by the machine to perform
method steps for calibration of polynucleotide sequence data, said method steps comprising:
(i) receiving a set of fluorescent emission intensity signals where each of said fluorescent emission intensity signals of
said set is comprised of a plurality of component signals, with each of said component signals representing a respective nucleotide
base or an internal standard in a multicomponent mixture including a plurality of analyte polynucleotide fragments of unknown
nucleotide sequence;
(ii) determining a first component signal corresponding to the internal standard;
(iii) determining a second, third, fourth and fifth component signal representing the respective nucleotide bases;
(iv) identifying features from said first component signal and determining at least one peak-shape characteristic thereof,
(v) generating a calibration model based at least in part on said at least one characteristic, and applying said calibration
model to said second, third, fourth and fifth component signals, and
(vi) reporting the result of applying said calibration model to said second, third, forth and fifth component signals to a
user.
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