| US 7,572,610 B2 | ||
| Methods of making compositions comprising a UV-Absorbing chromophore | ||
| Joseph A. Laszlo, Peoria, Ill. (US); David L. Compton, Peoria, Ill. (US); Louis J. DeFilippi, Palatine, Ill. (US); and Steven Grall, Lemont, Ill. (US) | ||
| Assigned to Biotechnology Research & Development Corporation, Peoria, Ill. (US); and The United States of America as represented by the Department of Agriculture, Washington, D.C. (US) | ||
| Filed on Jun. 19, 2006, as Appl. No. 11/425,096. | ||
| Claims priority of provisional application 60/723209, filed on Oct. 03, 2005. | ||
| Prior Publication US 2007/0077636 A1, Apr. 05, 2007 | ||
| Int. Cl. C12P 7/64 (2006.01) | ||
| U.S. Cl. 435—134 | 24 Claims |
1. A method of making a compound having the structural formula:
 wherein at least two of R1, R2, and R3 are non-fatty acid carboxylates; and
the other of R1, R2, and R3 are each either a C2-C24 fatty acid moiety, OH, or a non-fatty acid carboxylate, wherein the first and second non-fatty acid carboxylates are the same
or different and wherein at least one R1, R2, and R3 comprises a phytochemical, comprising:
(a) partially deacylating a triacylglycerol so as to provide a mono- or diacylglycerol wherein the triglycerol is a vegetable
oil selected from the group consisting of soybean oil, corn oil, sunflower seed oil, high-oleic sunflower seed oil, canola
oil, safflower oil, cuphea oil, coconut oil, olive oil, palm kernel oil and combinations thereof;
(b) reacting in a reaction mixture an acyl ester of the phytochemical with said mono- or diacylglycerol in the presence of
a esterase catalyst under conditions that permit transesterification of said ester with said mono- or diacylglycerol;
(c) recovering said compound from said reaction mixture; and
(d) removing volatile compounds from the reaction mixture prior to or following recovering said compound from said reaction
mixture.
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