| US 7,550,563 B2 | ||
| Method for the purification of a non-immunoglobulin protein comprising an immunoglobulin-like (Ig-like) domain | ||
| Boris Schwartsburd, Rehovot (Israel); and Ilana Belzer, Rishon-Le-Zion (Israel) | ||
| Assigned to Laboratoires Serono SA, (Switzerland) | ||
| Appl. No. 10/567,422 PCT Filed Aug. 05, 2004, PCT No. PCT/IL2004/000732 § 371(c)(1), (2), (4) Date Sep. 08, 2006, PCT Pub. No. WO2005/014621, PCT Pub. Date Feb. 17, 2005. |
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| Claims priority of application No. 157309 (IL), filed on Aug. 07, 2003. | ||
| Prior Publication US 2007/0032638 A1, Feb. 08, 2007 | ||
| Int. Cl. C07K 14/00 (2006.01); C12N 9/02 (2006.01) | ||
| U.S. Cl. 530—350 [530/351; 530/344; 435/189] | 18 Claims |
| 1. A method for purifying or capturing a non-immunoglobulin protein of interest having between one and ten immunoglobulin-like
(Ig-like) domains from a biological fluid, comprising the steps of:
a) contacting the biological fluid containing the protein of interest with a Hydrophobic Charge Induction Chromatography (HCIC)
resin, wherein the HCIC resin comprises a mercapto-ethyl pyridine ligand,
b) washing out the resin with a buffer to remove unbound contaminants, and
c) eluting the protein of interest by treating the resin with a buffer solution comprising an organic solvent, wherein the
organic solvent is isopropyl alcohol, propylene glycol and/or polyalcohols.
|