| US 7,514,243 B2 | ||
| Process for producing dipeptides | ||
| Shin-ichi Hashimoto, Hofu (Japan); and Kazuhiko Tabata, Machida (Japan) | ||
| Assigned to Kyowa Hakko Kogyo Co., Ltd., Tokyo (Japan) | ||
| Filed on Jun. 24, 2005, as Appl. No. 11/165,211. | ||
| Prior Publication US 2005/0287626 A1, Dec. 29, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12P 1/00 (2006.01); C12P 13/00 (2006.01); C12P 7/40 (2006.01) | ||
| U.S. Cl. 435—128 [435/41; 435/136] | 7 Claims |
| 1. A process for producing a dipeptide represented by formula (I):
R1—R2 (I)
wherein R1 is L-alanine, glycine, L-methionine, L-serine, L-threonine, β-alanine, L-cysteine or L-α-aminobutyric acid, and R2 is L-alanine, L-glutamine, L-glutamic acid, glycine, L-leucine, L-isoleucine, L-proline, L-tryptophan, L-methionine, L-serine,
L-theanine, L-cysteine, L-asparagine, L-lysine, L-arginine, L-histidine, L-aspartic acid, L-α-aminobutyric acid, , β-alanine,
L-azaserine, L-threonine, L-4-hydroxyproline, L-3-hydroxyproline, L-ornithine, L-citrulline or L-6-diazo-5-oxo-norleucine,
provided that both R1 and R2 be L-alanine at the same time, said method comprising:
culturing in a medium a microorganism which produces a protein having the amino acid sequence of SEQ ID NO: 1, or a protein
consisting of an amino acid sequence which has 95% or more homology to the amino acid sequence of SEQ ID NO: 1 and having
the activity of forming a dipeptide from one or more amino acids, and which has the ability to produce at least one of said
one or more of said amino acids;
allowing the dipeptide to form and accumulate in the medium; and
recovering the dipeptide from the medium.
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